Cypex - in vitro drug metabolism systems
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Cypex beagle dog CYP1A2 QC Assays

Activity: 7-ethoxycoumarin O-deethylase

Incubation conditions
Assay buffer: 0.05 M potassium phosphate pH 7.4, 5 mM MgCl2
Incubation volume: 200 µl
Stop reagent: 1 M hydrochloric acid (20 µl)
Internal standard: 4-methylumbelliferone (10 µl of 0.1 µg/ml)

HPLC conditions
Column: Hypersil ODS (5 µm) 250 x 4 mm
Temperature: 30
°C
Mobile phase: 0.05% orthophosphoric acid/acetonitrile (gradient separation)
Flow rate: 1.0 ml/min
Run time: 14.5 min
Injection volume: 50 µl
Detection: fluorescence
l(ex) = 324 nm, l(em) = 458 nm
Retention times:

       umbelliferone                5.2 min
     4-methylumbelliferone    7.0 min
     7-ethoxycoumarin         10.3 min

 

Important: By purchasing this product from Cypex you accept the standard terms and conditions of supply, details of which may be provided upon request.

 

Made under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other patents pending).

United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No. 2100245 and other patents pending.

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